Homogenized samples blended with magnetic beads; beads are washed with wash buffers, and RNA is then eluted from the beads Whilst we normally notice a pellet right after centrifugation, the majority on the protein continues to be within the supernatant. : Using the software program in your qPCR thermocycler, figure https://lingjunbio.quora.com/Properties-of-DNA-RNA-extraction-reagents-DNA-RNA-extraction-reagents-usually-include-lysis-buffer-proteinase-K-preci